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bug: Different heights when calling the same height of a labware with different pipettes (v7.0.1) #14416
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I experience the same bug/ a very, very similar bug. What happens: I then setup up the OT-2, and begin the protocol. However, as the non-off-setting pipette try to aspirate or dispense, it want to go further down than the labware is position, aka it crashes with the bottom of the labware (the P200 tips gets bend). Beside seeing labware the pressure on the labware, I also double check if the labware is movable - it is not, aka it is pinned. The error happens regardless if it is a p10+p50 setup or a p10+m20 setup. Background information Setup How have I tried to solve it? Thoughts |
Thank you both for the report. I'm not sure if the two of you are suffering from exactly the same combination of underlying problems, but here are some likely culprits. First: In general, any time there's a problem where one pipette works but the other doesn't, I'd take a look at pipette offset calibration and tip length calibration. (It sounds like @KU-JonasL has already done this.) Second, and probably more importantly, we've identified a bug that causes the robot to apply tip length calibration inconsistently, which can cause all kinds of mayhem. This will be fixed in v7.2.0. (PR #14512, internal ticket RSS-468.) It's difficult to confirm if this is totally responsible for the errors you're noticing, but if you've redone tip length and pipette offset calibration and you're still seeing this, it might have something to do with it. |
I'm going to close this because GitHub tends to not be a good venue for troubleshooting positioning and calibration problems. If you still have this problem after we release v7.2.0, I would contact Opentrons Support. They can help discern between, say, a software bug (which we can help with here) vs. a hardware problem with your robot (which we can't). Thanks again! |
Overview
Calibrating a labware with a P20S and running a protocol with a P20 while calling the pipette to move at 1mm from the bottom will give off a correct result i.e. pipette will move at 1mm from the labware's bottom.
However, in the same protocol incorporating a P300S and calling the pipette to move at 1mm of the labware will give off a wrong result by about -1.5mm i.e. the pipette will move at -0.5 from the labware's bottom.
This causes major inconsistencies in ones protocol especially if you are working with small volumes. In addition, this means that to overcome this one should incorporate the difference in the protocol something that is not ideal at all.
Steps to reproduce
See overview
Current behavior
No response
Expected behavior
No response
Operating system
Windows
System and robot setup or anything else?
OT-2R
App/server: v7.0.1
Wi-Fi
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