diff --git a/emulation_system/tests/e2e/results/ot3_results.py b/emulation_system/tests/e2e/results/ot3_results.py
index 2a8bdc63..587dc4d1 100644
--- a/emulation_system/tests/e2e/results/ot3_results.py
+++ b/emulation_system/tests/e2e/results/ot3_results.py
@@ -3,6 +3,8 @@
 from dataclasses import dataclass
 from typing import Set, Type
 
+from docker.models.containers import Container  # type: ignore[import]
+
 from tests.e2e.consts import (
     ENTRYPOINT_MOUNT,
     MONOREPO_WHEEL_VOLUME,
@@ -301,6 +303,42 @@ def get_actual_results(
         )
 
 
+@dataclass
+class PipetteEeproms(ResultABC):
+    """Validate that pipette eeproms are correct."""
+
+    left_pipette_eeprom_file_has_content: bool
+    right_pipette_eeprom_file_has_content: bool
+
+    @staticmethod
+    def _file_has_content(container: Container) -> bool:
+        return int(exec_in_container(container, "stat -c %s /eeprom/eeprom.bin")) > 0
+
+    @classmethod
+    def get_expected_results(
+        cls: Type["PipetteEeproms"], system_test_def: SystemTestDefinition
+    ) -> "PipetteEeproms":
+        """Generate expected pipette eeproms."""
+        return cls(
+            left_pipette_eeprom_file_has_content=system_test_def.left_pipette_expected,
+            right_pipette_eeprom_file_has_content=system_test_def.right_pipette_expected,
+        )
+
+    @classmethod
+    def get_actual_results(
+        cls: Type["PipetteEeproms"], system_under_test: E2EHostSystem
+    ) -> "PipetteEeproms":
+        """Load actual pipette eeproms."""
+        return cls(
+            left_pipette_eeprom_file_has_content=cls._file_has_content(
+                system_under_test.ot3_containers.left_pipette
+            ),
+            right_pipette_eeprom_file_has_content=cls._file_has_content(
+                system_under_test.ot3_containers.right_pipette
+            ),
+        )
+
+
 @dataclass
 class OT3Result(ResultABC):
     """Collect all ot3 result classes into a single dataclass."""
@@ -311,6 +349,7 @@ class OT3Result(ResultABC):
     emulator_mounts: OT3EmulatorMounts
     builder_named_volumes: OT3FirmwareBuilderNamedVolumes
     binaries: OT3Binaries
+    pipette_eeproms: PipetteEeproms
 
     @classmethod
     def get_actual_results(
@@ -330,6 +369,7 @@ def get_actual_results(
                 system_under_test
             ),
             binaries=OT3Binaries.get_actual_results(system_under_test),
+            pipette_eeproms=PipetteEeproms.get_actual_results(system_under_test),
         )
 
     @classmethod
@@ -350,4 +390,5 @@ def get_expected_results(
                 system_test_def
             ),
             binaries=OT3Binaries.get_expected_results(system_test_def),
+            pipette_eeproms=PipetteEeproms.get_expected_results(system_test_def),
         )
diff --git a/emulation_system/tests/e2e/system_mappings.py b/emulation_system/tests/e2e/system_mappings.py
index fce0b10d..c90d19bd 100644
--- a/emulation_system/tests/e2e/system_mappings.py
+++ b/emulation_system/tests/e2e/system_mappings.py
@@ -25,6 +25,8 @@
     ot3_firmware_build_args=BuildArgConfigurations.LATEST_BUILD_ARGS,
     opentrons_modules_build_args=BuildArgConfigurations.NO_BUILD_ARGS,
     module_configuration=ModuleConfiguration.NO_MODULES(),
+    left_pipette_expected=True,
+    right_pipette_expected=False,
 )
 
 OT3_FIRMWARE_DEV = SystemTestDefinition(
@@ -42,6 +44,8 @@
     ot3_firmware_build_args=BuildArgConfigurations.LATEST_BUILD_ARGS,
     opentrons_modules_build_args=BuildArgConfigurations.NO_BUILD_ARGS,
     module_configuration=ModuleConfiguration.NO_MODULES(),
+    left_pipette_expected=False,
+    right_pipette_expected=True,
 )
 
 OT3_AND_MODULES = SystemTestDefinition(
@@ -64,6 +68,8 @@
         fw_magnetic_module_names={"ot3-and-modules-magdeck"},
         fw_temperature_module_names={"ot3-and-modules-tempdeck"},
     ),
+    left_pipette_expected=True,
+    right_pipette_expected=True,
 )
 
 _TEST_DEFS: Dict[str, SystemTestDefinition] = {
diff --git a/emulation_system/tests/e2e/test_definition/system_test_definition.py b/emulation_system/tests/e2e/test_definition/system_test_definition.py
index c2ca3f16..bd3701e9 100644
--- a/emulation_system/tests/e2e/test_definition/system_test_definition.py
+++ b/emulation_system/tests/e2e/test_definition/system_test_definition.py
@@ -67,3 +67,5 @@ class SystemTestDefinition:
     opentrons_modules_build_args: BuildArgConfigurations
 
     module_configuration: ModuleConfiguration
+    left_pipette_expected: bool
+    right_pipette_expected: bool
diff --git a/samples/ci/ot3/ot3_remote.yaml b/samples/ci/ot3/ot3_remote.yaml
index 6b748516..de84b2f7 100644
--- a/samples/ci/ot3/ot3_remote.yaml
+++ b/samples/ci/ot3/ot3_remote.yaml
@@ -20,7 +20,6 @@ robot:
   emulation-level: hardware
   hardware-specific-attributes:
     left-pipette: P1000 Multi
-    right-pipette: P50 Multi
 
 monorepo-source: latest
 ot3-firmware-source: latest
diff --git a/samples/ci/team_specific_setups/ot3_firmware_development.yaml b/samples/ci/team_specific_setups/ot3_firmware_development.yaml
index 724976be..840f0d1e 100644
--- a/samples/ci/team_specific_setups/ot3_firmware_development.yaml
+++ b/samples/ci/team_specific_setups/ot3_firmware_development.yaml
@@ -19,7 +19,6 @@ robot:
   hardware: ot3
   emulation-level: hardware
   hardware-specific-attributes:
-    left-pipette: P1000 Multi
     right-pipette: P50 Multi
 
 monorepo-source: /home/runner/work/opentrons-emulation/opentrons-emulation/opentrons