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The decision to flip indices needs to be weighted by the amount of reads with "unknown" barcodes. For instance currently it can decide to flip I7 if it will go from 1 read to 10 reads demuxed, whereas there might be 100k reads with unknown.
Edit: for my own reference, see run 20240118_1039_MN19414_ASG790_ae66b218
The text was updated successfully, but these errors were encountered:
This shows out of the four possible orientations: original, revcomp i7, revcomp i5 and revcomp both it did not make the obvious choice which would yield many more matched reads.
The decision to flip indices needs to be weighted by the amount of reads with "unknown" barcodes. For instance currently it can decide to flip I7 if it will go from 1 read to 10 reads demuxed, whereas there might be 100k reads with unknown.
Edit: for my own reference, see run
20240118_1039_MN19414_ASG790_ae66b218
The text was updated successfully, but these errors were encountered: