Iso Seq Public Datasets

Last Updated: 2021/09/07

• Human & Mouse - single-cell
• Human - whole transcriptome
• Human - targeted
• Plant & Animal

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Human & Mouse - Single-Cell Iso-Seq

(1) Post-natal mouse brain

Key	Detail
Species	Mouse, postnatal brain
Sequencing	10X --> Sequel II
Analysis	Iso-Seq & R code
Data	GEO:GSE158450

Joglekar et al., "A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain", Nature Communications (2021)

(2) Mouse C2C12 cells (ENCODE project)

Key	Detail
Species	Mouse, C2C12 cells
Sequencing	Parse(SplitSeq) --> Sequel II
Analysis	Iso-Seq & R code
Data	GEO:GSE168776

Rebboah et al., "Mapping and modeling the genomic basis of differential RNA isoform expression at single-cell resolution with LR-Split-seq", biorxiv (2021)

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Human - Whole Transcriptome

(1) Universal Human Reference RNA (UHRR), 2021 Release

Key	Detail
Species	Human, UHRR
Sequencing	Sequel II
Analysis	Iso-Seq
Data	UHRRisoseq2021
Browser	UCSC Genome Browser Track

This is PacBio's internal UHRR data release.

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Plant & Animal

(1) Arabidopsis thaliana

Key	Detail
Species	A. thaliana, various tissues
Sequencing	Sequel I
Analysis	Iso-Seq & TAMA
Data	SRA PRJNA755474

Reference: Zhang et al. "A high resolution single molecule sequencing-based Arabidopsis transcriptome using novel methods of Iso-seq analysis", biorxiv (2021)