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Heatmap bugs #71

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kosankem opened this issue May 25, 2021 · 2 comments
Open

Heatmap bugs #71

kosankem opened this issue May 25, 2021 · 2 comments

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@kosankem
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There are no lables for the genes in the heatmap

@kosankem
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kosankem commented May 25, 2021

I can chose the setting RNAintegrated (assay) and e.g. data (slot) or counts (slot). However, the result are not shown/do not change, but instead the heatmap only displays the results of the last settings that I did with RNA (assay). Means, the heatmap does not work for the RNAintegrated assay, but I do not get any error indicating this.

Amendment: I think that above described problem is due to the fact that for the counts at least there are no data available for RNAintegrated: Error in Seurat::DoHeatmap(sc(), features = unlist(strsplit(input$genes, : No requested features found in the counts slot for the RNAintegrated assay.
In that case, it would be better to show no heatmap instead of the previously generated one. Moreover, if, in general, RNAintegrated has not a slot for counts (I think so, but I need to check) it is confusing to have it as a choice.

@kosankem
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In general, I think normally you would generate the heatmap with the scaled data which should be stored in the scale.data slot. I am not sure that it makes much sense to offer the possiblity to show the not-normalized and not-scaled data at all as in the most cases it would only lead to confusion or missinterpretation of data.

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