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Installed ngmaster 1.0.0 via bioconda using mamba:
# create conda env
$ mamba create -n ngmaster -c conda-forge -c bioconda -c defaults ngmaster
# activate env
$ mamba activate ngmaster
# test runs successfully
$ ngmaster --test
Running ngmaster.py on test example (NG-MAST 4186 / NG-STAR 231) ...
FILE SCHEME NG-MAST/NG-STAR porB_NG-MAST tbpB penA mtrR porB_NG-STAR ponA gyrA parC 23S
/home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/test/test.fa ngmaSTar 4186/231 2569 241 23 42 100 100 10 2 100
... Test successful.
# runs successfully on a N. gonnorhoeae genome
$ ngmaster contigs.fasta
FILE SCHEME NG-MAST/NG-STAR porB_NG-MAST tbpB penA mtrR porB_NG-STAR ponA gyrA parC 23S
contigs.fasta ngmaSTar 19265/2050 11156 362 23 19 4 1 100 2 100
# fails upon adding --minid
$ ngmaster --minid 85 contigs.fasta
Command '['/home/curtis_kapsak/miniconda3/envs/ngmaster/bin/mlst', '--legacy', '-q', '--threads', '16', '--datadir', '/home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/db/pubmlst', '--blastdb', '/home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/db/blast/mlst.fa', '--scheme', 'ngmast', '--minid', "['85']", '--mincov', '10', 'contigs.fasta']' returned non-zero exit status 1.
# fails upon adding --mincov
$ ngmaster --mincov 15 contigs.fasta
Command '['/home/curtis_kapsak/miniconda3/envs/ngmaster/bin/mlst', '--legacy', '-q', '--threads', '16', '--datadir', '/home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/db/pubmlst', '--blastdb', '/home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/db/blast/mlst.fa', '--scheme', 'ngmast', '--minid', '95', '--mincov', "['15']", 'contigs.fasta']' returned non-zero exit status 1.
# if I run the mlst cmd presented in the error, it runs successfully?
$ /home/curtis_kapsak/miniconda3/envs/ngmaster/bin/mlst --legacy -q --threads 16 --datadir /home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/db/pubmlst --blastdb /home/curtis_kapsak/miniconda3/envs/ngmaster/lib/python3.9/site-packages/ngmaster/db/blast/mlst.fa --scheme ngmast --minid 95 --mincov 15 contigs.fasta
FILE SCHEME ST porB tbpB
Warning: [blastn] Number of threads was reduced to 8 to match the number of available CPUs
contigs.fasta ngmast 19265 11156 362
# exit code is 0 for mlst command
$ echo$?
0
I've also installed ngmaster via pip and encountered the same issue too, so I think the issue is something specific to taking in those 2 optional inputs
Am I doing something wrong here?
The text was updated successfully, but these errors were encountered:
Installed ngmaster 1.0.0 via bioconda using
mamba
:I've also installed ngmaster via
pip
and encountered the same issue too, so I think the issue is something specific to taking in those 2 optional inputsAm I doing something wrong here?
The text was updated successfully, but these errors were encountered: