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Fail with status: 137 #7

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jnarayan81 opened this issue May 24, 2017 · 0 comments
Open

Fail with status: 137 #7

jnarayan81 opened this issue May 24, 2017 · 0 comments

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@jnarayan81
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jnarayan81 commented May 24, 2017

This use to be fine on my UBUNTU, but now it fail with status: 137.
I tested it on 9MB data with 256GB RAM but killed :( ... I notice, it consume all RAM and perhaps ask for more.

urbe@urbo214b[MeGAMerge] perl MeGAMerge-1.1vimal.pl corrected_MIRA_SPACES_all allCorrected.fa -d -force []
COMMAND
perl MeGAMerge-1.1vimal.pl corrected_MIRA_SPACES_all allCorrected.fa -d -force
The Merged FASTA will be stored in corrected_MIRA_SPACES_all/MergedContigs.fasta
Reading allCorrected.fa
Running Newbler assembly with 566 sequences
runAssembly -force -large -rip -mi 98 -ml 80 -pairt -cpu 40 -a 0 -o corrected_MIRA_SPACES_all/newbler corrected_MIRA_SPACES_all/newblerIn.fasta
Created assembly project directory corrected_MIRA_SPACES_all/newbler
1 read file successfully added.
newblerIn.fasta (Fasta dataset)
Assembly computation starting at: Tue May 23 16:25:54 2017 (v2.9 (20130529_1641))
Indexing newblerIn.fasta...
Warning: No quality scores file found.
-> 566 reads, 257471 bases.
Setting up long overlap detection...
-> 565 of 565, 530 reads to align
Building a tree for 5108 seeds...
Computing long overlap alignments...

4 sequences marked as repeats, 4 in alignments, removing from 5 chords...
-> 565 of 565
Setting up overlap detection...
-> 565 of 565, 556 reads to align
Starting seed building...
-> 565 of 565
Building a tree for 20289 seeds...
Computing alignments...

5 sequences marked as repeats, 1 in alignments, removing from 3 chords...
-> 565 of 565
Checkpointing...
Detangling alignments...
-> Level 4, Phase 9, Round 1...
Checkpointing...
Building contigs/scaffolds...
-> 4 large contigs, 10 all contigs
Computing signals...
-> 7429 of 7429...
Generating output...
-> 7429 of 7429...
Assembly computation succeeded at: Tue May 23 16:25:56 2017
517 newbler singletons sequences
Checking 454PairAlign.txt
Unique Newbler Singletons (>=0 bp): 503
opening corrected_MIRA_SPACES_all/minimus.fasta for writing
opening corrected_MIRA_SPACES_all/largefile.fasta for reading
opening corrected_MIRA_SPACES_all/newbler/All.fasta for reading
Running Minimus2 with 568 sequences
Max ID: 1707
minimus2 -D CONSERR=0.06 -D MINID=98 -D OVERLAP=80 corrected_MIRA_SPACES_all/minimus.fasta
The log file is: corrected_MIRA_SPACES_all/minimus.fasta.runAmos.log
Doing step 10: Building AMOS bank & Dumping reads
Doing step 11
Doing step 12
Doing step 13
Doing step 20: Getting overlaps
Doing step 21
Doing step 22
Doing step 23: Converting overlaps
Doing step 24: Loading overlaps to the bank
Doing step 25
Doing step 30: Running contigger
Doing step 31
Doing step 40: Running consensus
Doing step 41
Command: /home/urbe/Tools/amos-3.1.0/bin/make-consensus -B -e 0.06 -b corrected_MIRA_SPACES_all/minimus.fasta.bnk -w 15 exited with status: 137
minimus2 -D CONSERR=0.06 -D MINID=98 -D OVERLAP=80 corrected_MIRA_SPACES_all/minimus.fasta failed:
Died at MeGAMerge-1.1vimal.pl line 283.

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