You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
This use to be fine on my UBUNTU, but now it fail with status: 137.
I tested it on 9MB data with 256GB RAM but killed :( ... I notice, it consume all RAM and perhaps ask for more.
urbe@urbo214b[MeGAMerge] perl MeGAMerge-1.1vimal.pl corrected_MIRA_SPACES_all allCorrected.fa -d -force []
COMMAND
perl MeGAMerge-1.1vimal.pl corrected_MIRA_SPACES_all allCorrected.fa -d -force
The Merged FASTA will be stored in corrected_MIRA_SPACES_all/MergedContigs.fasta
Reading allCorrected.fa
Running Newbler assembly with 566 sequences
runAssembly -force -large -rip -mi 98 -ml 80 -pairt -cpu 40 -a 0 -o corrected_MIRA_SPACES_all/newbler corrected_MIRA_SPACES_all/newblerIn.fasta
Created assembly project directory corrected_MIRA_SPACES_all/newbler
1 read file successfully added.
newblerIn.fasta (Fasta dataset)
Assembly computation starting at: Tue May 23 16:25:54 2017 (v2.9 (20130529_1641))
Indexing newblerIn.fasta...
Warning: No quality scores file found.
-> 566 reads, 257471 bases.
Setting up long overlap detection...
-> 565 of 565, 530 reads to align
Building a tree for 5108 seeds...
Computing long overlap alignments...
4 sequences marked as repeats, 4 in alignments, removing from 5 chords...
-> 565 of 565
Setting up overlap detection...
-> 565 of 565, 556 reads to align
Starting seed building...
-> 565 of 565
Building a tree for 20289 seeds...
Computing alignments...
5 sequences marked as repeats, 1 in alignments, removing from 3 chords...
-> 565 of 565
Checkpointing...
Detangling alignments...
-> Level 4, Phase 9, Round 1...
Checkpointing...
Building contigs/scaffolds...
-> 4 large contigs, 10 all contigs
Computing signals...
-> 7429 of 7429...
Generating output...
-> 7429 of 7429...
Assembly computation succeeded at: Tue May 23 16:25:56 2017
517 newbler singletons sequences
Checking 454PairAlign.txt
Unique Newbler Singletons (>=0 bp): 503
opening corrected_MIRA_SPACES_all/minimus.fasta for writing
opening corrected_MIRA_SPACES_all/largefile.fasta for reading
opening corrected_MIRA_SPACES_all/newbler/All.fasta for reading
Running Minimus2 with 568 sequences
Max ID: 1707
minimus2 -D CONSERR=0.06 -D MINID=98 -D OVERLAP=80 corrected_MIRA_SPACES_all/minimus.fasta
The log file is: corrected_MIRA_SPACES_all/minimus.fasta.runAmos.log
Doing step 10: Building AMOS bank & Dumping reads
Doing step 11
Doing step 12
Doing step 13
Doing step 20: Getting overlaps
Doing step 21
Doing step 22
Doing step 23: Converting overlaps
Doing step 24: Loading overlaps to the bank
Doing step 25
Doing step 30: Running contigger
Doing step 31
Doing step 40: Running consensus
Doing step 41
Command: /home/urbe/Tools/amos-3.1.0/bin/make-consensus -B -e 0.06 -b corrected_MIRA_SPACES_all/minimus.fasta.bnk -w 15 exited with status: 137
minimus2 -D CONSERR=0.06 -D MINID=98 -D OVERLAP=80 corrected_MIRA_SPACES_all/minimus.fasta failed:
Died at MeGAMerge-1.1vimal.pl line 283.
The text was updated successfully, but these errors were encountered:
This use to be fine on my UBUNTU, but now it fail with status: 137.
I tested it on 9MB data with 256GB RAM but killed :( ... I notice, it consume all RAM and perhaps ask for more.
urbe@urbo214b[MeGAMerge] perl MeGAMerge-1.1vimal.pl corrected_MIRA_SPACES_all allCorrected.fa -d -force []
COMMAND
perl MeGAMerge-1.1vimal.pl corrected_MIRA_SPACES_all allCorrected.fa -d -force
The Merged FASTA will be stored in corrected_MIRA_SPACES_all/MergedContigs.fasta
Reading allCorrected.fa
Running Newbler assembly with 566 sequences
runAssembly -force -large -rip -mi 98 -ml 80 -pairt -cpu 40 -a 0 -o corrected_MIRA_SPACES_all/newbler corrected_MIRA_SPACES_all/newblerIn.fasta
Created assembly project directory corrected_MIRA_SPACES_all/newbler
1 read file successfully added.
newblerIn.fasta (Fasta dataset)
Assembly computation starting at: Tue May 23 16:25:54 2017 (v2.9 (20130529_1641))
Indexing newblerIn.fasta...
Warning: No quality scores file found.
-> 566 reads, 257471 bases.
Setting up long overlap detection...
-> 565 of 565, 530 reads to align
Building a tree for 5108 seeds...
Computing long overlap alignments...
4 sequences marked as repeats, 4 in alignments, removing from 5 chords...
-> 565 of 565
Setting up overlap detection...
-> 565 of 565, 556 reads to align
Starting seed building...
-> 565 of 565
Building a tree for 20289 seeds...
Computing alignments...
5 sequences marked as repeats, 1 in alignments, removing from 3 chords...
-> 565 of 565
Checkpointing...
Detangling alignments...
-> Level 4, Phase 9, Round 1...
Checkpointing...
Building contigs/scaffolds...
-> 4 large contigs, 10 all contigs
Computing signals...
-> 7429 of 7429...
Generating output...
-> 7429 of 7429...
Assembly computation succeeded at: Tue May 23 16:25:56 2017
517 newbler singletons sequences
Checking 454PairAlign.txt
Unique Newbler Singletons (>=0 bp): 503
opening corrected_MIRA_SPACES_all/minimus.fasta for writing
opening corrected_MIRA_SPACES_all/largefile.fasta for reading
opening corrected_MIRA_SPACES_all/newbler/All.fasta for reading
Running Minimus2 with 568 sequences
Max ID: 1707
minimus2 -D CONSERR=0.06 -D MINID=98 -D OVERLAP=80 corrected_MIRA_SPACES_all/minimus.fasta
The log file is: corrected_MIRA_SPACES_all/minimus.fasta.runAmos.log
Doing step 10: Building AMOS bank & Dumping reads
Doing step 11
Doing step 12
Doing step 13
Doing step 20: Getting overlaps
Doing step 21
Doing step 22
Doing step 23: Converting overlaps
Doing step 24: Loading overlaps to the bank
Doing step 25
Doing step 30: Running contigger
Doing step 31
Doing step 40: Running consensus
Doing step 41
Command: /home/urbe/Tools/amos-3.1.0/bin/make-consensus -B -e 0.06 -b corrected_MIRA_SPACES_all/minimus.fasta.bnk -w 15 exited with status: 137
minimus2 -D CONSERR=0.06 -D MINID=98 -D OVERLAP=80 corrected_MIRA_SPACES_all/minimus.fasta failed:
Died at MeGAMerge-1.1vimal.pl line 283.
The text was updated successfully, but these errors were encountered: