Can't open splitrim stats: No such file or directory

[Coaxecva]

Hi,

I got this error when I ran with the option "--input file_1.fastq,file_2.fastq" :

[00:00:00] Starting GOTTCHA v1.0c
[00:00:00] Auto set database level to SPECIES.
[00:00:00] Number of threads: 8
[00:00:01] Checking running environment...
[00:00:01] Done. All required scripts and tools found.
[00:00:01] Split-trimming with parameters fixL=30, minQ=20, ascii=33.
[00:00:01] Split-trimming: /file_1.fastq...
[00:02:56] Done splitrimming /file_1.fastq.
[00:02:56] Split-trimming: ~/file_2.fastq...
[00:02:56] Done splitrimming ~/file_2.fastq.
Can't open splitrim stats: No such file or directory

It is running fine with one fastq file. Can you help to solve this?

Thanks,
Coax

[poeli]

Hi Coax,

The split-trim step finished immediately. I suspect there is something wrong with "file_2.fastq" (could be empty). Please double check.

Thanks,
Paul

[Coaxecva]

Thanks, Paul.

I think the software doesn't get this linux command "~/" . I used "--input ~/<path-to-my-folder>/file_1.fastq,~/<path-to-my-folder>/file_2.fastq". It can only recognize the first "~/".

Coax