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Input Stream Error during mergeLocusGraph #99

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VicBioDev opened this issue Aug 25, 2017 · 4 comments
Closed

Input Stream Error during mergeLocusGraph #99

VicBioDev opened this issue Aug 25, 2017 · 4 comments

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@VicBioDev
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VicBioDev commented Aug 25, 2017

Hi,

During a recent somatic run, input stream error was encountered while executing megerLocusGraph. One empty bin file under "svLocusGraph.bin.tmpdir" could be causing the problem as excluding the file from the list resolves the issue. Please check what caused the file to be empty, or if there is some other cause.

Files can be download here.

Error code listed below.

[2017-08-24T02:02:51.978624] [gb203] [15523_1] [TaskManager] [ERROR] Failed to complete command task: 'mergeLocusGraph' lauched from master workflow, error code: 1, command: '/home/Share/softwares/manta_1.1.1/libexec/MergeSVLoci --output-file /home/liyuan/analysis_temp/CtDNA_SJY23_male/manta/workspace/svLocusGraph.bin --graph-file-list /home/liyuan/analysis_temp/CtDNA_SJY23_male/manta/workspace/svLocusGraph.bin.tmpdir/list.svLocusGraph.txt'
[2017-08-24T02:02:51.980594] [gb203] [15523_1] [TaskManager] [ERROR] [mergeLocusGraph] Error Message:
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [mergeLocusGraph] Last 6 stderr lines from task (of 6 total lines):
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [2017-08-24T02:02:51.940472] [gb203] [15523_1] [mergeLocusGraph] FATAL_ERROR: EXCEPTION: input stream error
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [2017-08-24T02:02:51.941467] [gb203] [15523_1] [mergeLocusGraph] ...caught in program.run()
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [2017-08-24T02:02:51.942354] [gb203] [15523_1] [mergeLocusGraph] cmdline: /home/Share/softwares/manta_1.1.1/libexec/MergeSVLoci --output-file /home/liyuan/analysis_temp/CtDNA_SJY23_male/manta/workspace/svLocusGraph.bin --graph-file-list /home/liyuan/analysis_temp/CtDNA_SJY23_male/manta/workspace/svL ocusGraph.bin.tmpdir/list.svLocusGraph.txt
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [2017-08-24T02:02:51.943041] [gb203] [15523_1] [mergeLocusGraph] version: 1.1.1
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [2017-08-24T02:02:51.943712] [gb203] [15523_1] [mergeLocusGraph] buildTime: 2017-05-27T03:23:56.028559Z
[2017-08-24T02:02:51.982736] [gb203] [15523_1] [TaskManager] [ERROR] [2017-08-24T02:02:51.944368] [gb203] [15523_1] [mergeLocusGraph] compiler: g++-4.8.5
[2017-08-24T02:02:51.984443] [gb203] [15523_1] [TaskManager] [ERROR] Shutting down task submission. Waiting for remaining tasks to complete.

@ctsa
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ctsa commented Aug 25, 2017

Thanks for the report, we reproduce the issue here. We'll send an update when this is sorted.

@ctsa
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ctsa commented Aug 26, 2017

Note if you'd like a temporary workaround you can disable --callRegions. Also note (whether --callRegions is used or not) that for any type of targeted input data the --exome configuration option should be used to disable high depth filters.

ctsa added a commit that referenced this issue Aug 29, 2017
A bug was preventing the binary graph file from being written out by
EstimateSVLoci when multi-region is used and no candidate SV content
is found in the targeted regions. Fixed in this patch.

Closes #99.
@ctsa
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ctsa commented Aug 29, 2017

We've finished the fix. This will be available in the next release. For now I've backported the patch onto the v1.1.1 release code here:

https://github.com/Illumina/manta/tree/MANTA-1211-backport-for-v1.1.1

@VicBioDev
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@ctsa Thanks for the fix.

@x-chen x-chen closed this as completed in 8381b73 Sep 28, 2017
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