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In describing DNA assembly reactions, it would be useful to have a specific term for ligation of sequences.
It seems appropriate that this should be a child of SBO:conversion (SBO:0000182), as ligation is a modification of covalent bonds. It is also complementary with SBO:cleavage (SBO:0000178), which is the rupture of a covalent bond, whereas ligation is the formation of a covalent bond. It is also closely related to SBO:polymerization (SBO:0000652), except that refers to the joining of monomers to form a polymer whereas ligation is the joining of two or more polymers to form a larger polymer
Accordingly, I propose the following:
Name: ligation
Definition: Formation of a covalent bond resulting in the creation of a link between the ends of one or more linear polymer molecules.
Parent: SBO:conversion (SBO:0000182)
Note that this definition of ligation can apply not only to DNA ligation but also the RNA ligation and protein ligation. This definition also allows for ligation to describe circularization of a single DNA fragment, joining of two DNA fragments, and joining of multiple DNA fragments.
The text was updated successfully, but these errors were encountered:
In describing DNA assembly reactions, it would be useful to have a specific term for ligation of sequences.
It seems appropriate that this should be a child of
SBO:conversion
(SBO:0000182), as ligation is a modification of covalent bonds. It is also complementary withSBO:cleavage
(SBO:0000178), which is the rupture of a covalent bond, whereas ligation is the formation of a covalent bond. It is also closely related toSBO:polymerization
(SBO:0000652), except that refers to the joining of monomers to form a polymer whereas ligation is the joining of two or more polymers to form a larger polymerAccordingly, I propose the following:
SBO:conversion
(SBO:0000182)Note that this definition of ligation can apply not only to DNA ligation but also the RNA ligation and protein ligation. This definition also allows for ligation to describe circularization of a single DNA fragment, joining of two DNA fragments, and joining of multiple DNA fragments.
The text was updated successfully, but these errors were encountered: