Running Anvi'o interactive on a browser through Bigelow server.

Will need to open two terminals for this. The reference on the Anvi'o page is here, which we will follow with some tweaks: http://merenlab.org/2015/11/28/visualizing-from-a-server/

On first terminal, ssh into your account as normal, then start an interactive session:
qsub -I -q route -l walltime=0:80:00 -l ncpus=4,mem=4G -N jackie-interactive

then, load anvio

module use /mnt/scgc_nfs/opt/modulefiles/common
module load anaconda3
source activate anvio3
( Could also load anvi'o 4, see github page on running anvio)

navigate to the folder where your Anvi'o files are, and run the interactive:

anvi-interactive -p SAMPLES-MERGED/PROFILE.db -c AM_ALLmeta_Norm_sccare.db --server-only -P 8099 ( pick a port number if that one is taken or not working) You should see a screen that says the server is now listening to the port.

In second terminal: ssh -t -t [email protected] -L 8099:localhost:8099 ssh c1 -L 8099:localhost:8099 Replace with your own user ID and whatever port you chose. You may see an error "bind: Cannot assign requested address" Ignore it. Do not close this terminal window.

Go to any browser, and put in the address bar:

http://localhost:8099

(of course changing the ports to match above.